In our previous report, five different versions of Cantron and related formulas were tested for their scavenging abilities against the “Reactive Oxygen Species” of free radicals. This study includes 3 other variations of the formulation. Two of these variations (JVS-23 and EJS-50) were developed under the auspices of the children of James V. Sheridan. The other variation (TB) was developed by the consulting chemist of the Sheridan children. The JVS-23 formula has been reported to be the original Entelev formula and the EJS-50 has been reported to be the original Cancell formula. The TB formula has been called both Entelev and Cancell, but by all indication it is a variation of the Entelev formula. To obtain the full picture on all variations, it was deemed important to screen all three of these formulas for their antioxidant abilities as compared to the other variations that were previously studied. Nutraceutical chemistry consultant, Daniel Hetrick, PhD was in charge of the study.

ORAC and HORAC Testing of the Three Samples

In July of 2003, samples of the three formulas were sent to Brunswick laboratories. They were marked JVS- 23, EJS-50 and TB. The testing was completed and the report was compiled on 8/6/03 (report is available upon request).

As expected, the results prove that these versions were also very powerful peroxyl and hydroxyl radical scavengers. Rather unexpectedly, however, there was very little difference between these three formulas. In contrast to the proclamations of the Sheridan children and their consulting chemist, none of these formulas behaved anything like the original Entelev manufactured by James V. Sheridan or the original Cancell samples manufactured by Ed Sopcak, which were previously tested. Conclusion- All 3 variations were highly effective on hydroxyl and peroxyl radicals. EJS-50 and TB were virtually identical in their behavior. JVS-23 behaved marginally different and was slightly more effective than the other two. Of all the formulas tested in both studies, Cantron, New Millennium maintains the highest hydroxyl absorption capacity. The three formulas in this study have higher ORAC scores than New Millennium Cantron, but significantly lower ORAC values than the 1997Cantron, and the 1988 Cancell versions.

HORAC Scores

Superoxide radical scavenging ability of the three variant formulations

Samples of all three formulas were sent to Cayman laboratories to conduct superoxide dismutase assays. The results were mixed. The EJS-50 sample showed no effect on blocking superoxide radicals, TB showed a complete blocking effect, while JVS-23 had partial blocking effect.

Dr. Hetrick, however, advised after the study was completed, that the TB sample may have been highly concentrated due to evaporation (possibly inflating the results), and that the EJS-50 sample may have been diluted (possibly deflating the results). Therefore, these studies should be repeated utilizing better samples of TB and EJS-50.

Conclusion

JVS-23 showed partial scavenging ability of superoxide radicals in the essay. EJS-50 and TB need to be retested with better samples.

Table:Results for Superoxide Anion Scavenging in the SOD Assay (Cayman Laboratories)

Discussion of the Additional Studies

As was demonstrated in our previous study, there seems to be an inverse relationship or "seesaw" effect regarding HORAC and ORAC scores. There is a pattern in these studies, that when HORAC scores go up, ORAC scores go down. This phenomenon is manifested again when comparing Cantron, New Millennium, to the three variations tested in this report. The New Millennium version has the highest HORAC (45,003), compared to JVS-23 (39,071); while it had lower ORAC scores (1,081,886) to (1,400,984). JVS-23's ORAC scores, however, are still significantly lower than 1997 Cantron (2,122,527) and 1988 Cancell (1,882,618), but the latter's HORAC scores are much lower, complying with the "seesaw" effect.

Which formula is the best and most balanced antioxidant? One could make convincing arguments for either Cantron, New Millennium, or for JVS-23. It could be argued that because New Millennium Cantron had the highest HORAC scores while maintaining high ORAC scores that it is the best version, especially when considering that hydroxyl radicals are the most reactive and dangerous of all radicals. One can also argue that by scavenging more hydroxyl radicals it will also prevent further peroxyl radicals from forming (hydroxyls create peroxyls). Each hydroxyl radical that is allowed to exist without being scavenged can cause chain reactions and propagate thousands or even millions of additional peroxyl radicals. As Cantron was also a more effective superoxide radical scavenger, the argument for the new millennium version is bolstered even more. One could argue that because JVS-23 is higher in peroxyl scavenging abilities that it is the most effective. With that logic, however, one could make the argument that the 1997Cantron version is the most effective because it had the highest ORAC scores. Deciding which formula is the best is somewhat subjective, but it cannot be denied that both formulas are powerful and versatile antioxidants, which work to quench a large amount of hydroxyl, peroxyl and superoxide radicals.

The biggest surprise was how vastly different the Cancell formula was from EJS-50 which was proclaimed to be the Cancell equivalent. Cancell's HORAC is 215 vs. 1,332,821 for the EJS-50 while Cancell's ORAC is 1,882,618 to EJS-50's is 1,332,821. If they were equivalent formulas, they would behave the same. Therefore, it is apparent from these studies that EJS-50 is not Cancell. TB and EJS-50 are almost identical in their results; therefore, it is more likely that EJS-50 is the same formula as the TB formula. Likewise, JVS-23 behaved much differently than Sheridan's Entelev.

The 1982 Vintage Entelev formulation was the only sample available for testing that was actually manufactured by James V. Sheridan. It is important to note that Sheridan improved the formula in August 1984. This formula was not tested because a sample was not immediately available. In the future, MRP will manufacture this version according to Sheridan's 1984 specs and test it for comparison to all other versions.

 

Final Conclusion of the Additional Studies

All three variations tested in this study are extremely effective scavengers of peroxyl and hydroxyl radicals. The TB sample completely blocked formation of superoxide radicals in the assay, JVS-23 was partially effective, and EJS-50 had no effect. The superoxide-screening test for EJS- 50 and TB need to be repeated with better samples. Based on their behavior in these assays, it appears that EJS-50 and Cancell are different variations, while EJS-50 and TB appear to be the same. JVS-23 had slightly higher ORAC and HORAC scores than EJS-50 and TB.

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